Advancements In Mammalian Cell Culture Cell Culture Assays

What you'll learn
An overview of different mammalian cell culture assays in the field of biotechnology
Importance and applications of various cell culture assays
Introduction and basic principle of MTT assay
Detailed methodology/protocol of MTT assay (Day 1 to Day 3)
Results analysis of MTT assay
Introduction of Scratch assay / Wound healing assay
Complete methodology and results analysis of scratch assay
Introduction and importance of Antiviral assay
Two step methodology of Antiviral assay and complete protocol of both steps
Results analysis and overview
Requirements
This course needs some basic understanding of mammalian cell culture. You will acquire comprehensive knowledge regarding advancements in mammalian cell culture.
Description
This course is designed for undergraduate and graduate biotechnology students who possess an inclination towards acquiring knowledge in cell culture techniques but lack a starting point. This resource will primarily assist students with some basic background in mammalian cell culture. You will acquire comprehensive knowledge of different important cell culture assays and techniques. This course is designed for individuals who have a background in biotechnology, immunology, genetics, proteomics, cancer biology, microbiology, molecular biology, stem cell biology, cell and gene therapy, and animal cell culture.The purpose of this course is to provide an overview of the assays of mammalian cell culture. The initial lessons of the program provide a fundamental introduction to MTT assay (a critical technique in cell culture), including introduction, three-day protocol, and results analysis, as well as the significance and applications in the field of biotechnology. The second section introduces the wound healing or scratch assay, along with its detailed methodology and results analysis. The third section covers the introduction, importance, methodology, and analysis of Antiviral assay. Although there are certain similarities in the fundamentals of cell culture experiments, the conditions under which cells are cultured differ significantly between labs and cell types. Altering the culture conditions specified for a given cell line may lead to the expression of distinct phenotypes. Consequently, it is advisable that researchers acquaint themselves with the cell line of interest and diligently adhere to the instructions included with every product utilized in their experiments. (Note: The protocols provided in the course are performed by the instructor but can be changed according to the lab conditions.)
Overview
Section 1: Introduction
Lecture 1 Introduction to the course; Advancements in Mammalian Cell Culture | Assays
Section 2: MTT Assay
Lecture 2 Introduction of MTT Assay
Lecture 3 Methodology of MTT Assay ; Day 1
Lecture 4 Methodology of MTT Assay; Day 2
Lecture 5 Methodology of MTT Assay; Day 3
Lecture 6 Results Analysis of MTT Assay
Section 3: Scratch Assay | Wound Healing Assay
Lecture 7 Introduction of Wound Healing Assay
Lecture 8 Methodology and Analysis of Wound Healing Assay
Section 4: Antiviral Assay
Lecture 9 Introduction of Antiviral Assay and its Importance
Lecture 10 Methodology of Step 1 of Antiviral Assay
Lecture 11 Methodology of Step 2 of Antiviral Assay
Lecture 12 Results Analysis of Antiviral Assay
Section 5: Recap of the course
Lecture 13 Recap of Cell Culture Assays
Section 6: Resources
Lecture 14 Resources And References
This course is designed for undergraduate and graduate biotechnology students who possess some basics of mammalian cell culture and have an inclination towards acquiring knowledge in cell culture assays and techniques but lack a starting point. This resource will primarily assist students with a background in biology, particularly in the fields of biotechnology, molecular biology, cell biology, microbiology, genetics, proteomics, and related disciplines, in initiating their research projects.
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